1JST
PHOSPHORYLATED CYCLIN-DEPENDENT KINASE-2 BOUND TO CYCLIN A
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Detector technology | CCD |
| Collection date | 1995-12-22 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 139.600, 149.100, 74.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 7.000 - 2.600 |
| R-factor | 0.2 * |
| Rwork | 0.200 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.740 |
| Data reduction software | HKL |
| Data scaling software | SCALEPACK |
| Refinement software | TNT |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 * |
| High resolution limit [Å] | 2.600 * |
| Rmerge | 0.066 |
| Total number of observations | 290942 * |
| Number of reflections | 48448 * |
| Completeness [%] | 98.9 |
| Redundancy | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 * | 4 * | Jeffrey, P.D., (1995) Nature, 376, 313. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | CDK2 and cyclinA fragment | 25 (mg/ml) | |
| 2 | 1 | drop | HEPES | 40 (mM) | |
| 3 | 1 | drop | 0.2 (M) | ||
| 4 | 1 | drop | DTT | 5 (mM) | |
| 5 | 1 | reservoir | 28 (%sat) | ||
| 6 | 1 | reservoir | 1 (M) | ||
| 7 | 1 | reservoir | HEPES | 40 (mM) | |
| 8 | 1 | reservoir | DTT | 5 (mM) | |
| 9 | 1 | drop | ATP | 10 (mM) |
