1JSS
Crystal structure of the Mus musculus cholesterol-regulated START protein 4 (StarD4).
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9A |
| Synchrotron site | NSLS |
| Beamline | X9A |
| Temperature [K] | 150 |
| Detector technology | CCD |
| Collection date | 2001-05-25 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9791, 0.9795 |
| Spacegroup name | P 31 |
| Unit cell lengths | 69.966, 69.966, 85.190 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 * - 2.200 |
| R-factor | 0.234 * |
| Rwork | 0.238 |
| R-free | 0.28100 * |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.600 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.051 | 0.301 |
| Number of reflections | 46556 | |
| <I/σ(I)> | 28 | 6.8 |
| Completeness [%] | 98.5 | 98.3 |
| Redundancy | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 4 * | 11% PEG8000, 22% glycerol, 0.16M magnesium acetate, 0.08M sodium cacodylate pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | HEPES | 20 (mM) | pH7.5 |
| 2 | 1 | drop | 100 (mM) | ||
| 3 | 1 | drop | dithiothreitol | 5 (mM) | |
| 4 | 1 | drop | protein | 12 (mg/ml) | |
| 5 | 1 | reservoir | sodium cacodylate | 0.1 (M) | pH6.5 |
| 6 | 1 | reservoir | magnesium acetate | 0.2 (M) | |
| 7 | 1 | reservoir | PEG8000 | 16 (%(w/v)) | |
| 8 | 1 | reservoir | glycerol | 22 (%(v/v)) |
