1JSM

STRUCTURE OF H5 AVIAN HAEMAGGLUTININ

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Collection date	1999-12-31
Detector	ADSC QUANTUM 4
Wavelength(s)	1
Spacegroup name	H 3 2
Unit cell lengths	130.728, 130.728, 254.714
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.000 - 1.900
R-factor	0.234
Rwork	0.234
R-free	0.26400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009 *
RMSD bond angle	1.500 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000
High resolution limit [Å]	1.900	1.900
Rmerge	0.070 *	0.206 *
Number of reflections	55752	3643 *
Completeness [%]	84.4	56.4 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	MME-PEG 2000, NiCl2, HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	HEPES	10 (mM)
3	1	reservoir	PEG2000MME	20 (%)
4	1	reservoir		2 (mM)
5	1	reservoir	HEPES	100 (mM)