1JSF
FULL-MATRIX LEAST-SQUARES REFINEMENT OF HUMAN LYSOZYME
Experimental procedure
Source type | ROTATING ANODE |
Source details | ENRAF-NONIUS FR571 |
Temperature [K] | 285 |
Detector technology | AREA DETECTOR |
Collection date | 1996-01 |
Detector | ENRAF-NONIUS |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 57.050, 60.960, 32.960 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.900 - 1.150 |
R-factor | 0.112 |
R-free | 0.15700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1rex |
RMSD bond length | 0.014 |
Data reduction software | MERGEF |
Data scaling software | MERGEF |
Phasing software | SHELXL-93 |
Refinement software | SHELXL-93 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.100 | 1.160 |
High resolution limit [Å] | 1.140 | 1.140 |
Rmerge | 0.054 | 0.267 |
Number of reflections | 39326 | |
<I/σ(I)> | 6.2 | |
Completeness [%] | 92.1 | 43.8 |
Redundancy | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 4.5 | 3M AMMONIUM NITRATE SOLUTION WITH PH 4.5 ACETATE BUFFER AND 2% PROTEIN, VAPOR DIFFUSION OVER 5M AMMONIUM NITRATE AT ROOM TEMPERATURE. |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | ammonium nitrate | 3 (M) | |
2 | 1 | 1 | protein | 2 (%) | |
3 | 1 | 2 | ammonium nitrate | 5 (M) |