1JSE
FULL-MATRIX LEAST-SQUARES REFINEMENT OF TURKEY LYSOZYME
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR571 |
| Temperature [K] | 285 |
| Detector technology | AREA DETECTOR |
| Collection date | 1996-01 |
| Detector | ENRAF-NONIUS |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.150, 33.320, 46.240 |
| Unit cell angles | 90.00, 110.14, 90.00 |
Refinement procedure
| Resolution | 22.300 - 1.120 |
| R-factor | 0.103 |
| R-free | 0.14000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1LZ3 |
| RMSD bond length | 0.013 |
| Data reduction software | MERGEF |
| Data scaling software | MERGEF |
| Phasing software | SHELXL-93 |
| Refinement software | SHELXL-93 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.300 | 1.140 |
| High resolution limit [Å] | 1.120 | 1.120 |
| Rmerge | 0.043 | 0.227 |
| Number of reflections | 36320 | |
| <I/σ(I)> | 5.3 | |
| Completeness [%] | 86.1 | 53.3 |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 4.2 | 2.2M AMMONIUM SULFATE SOLUTION AT PH 4.2 CONTAINING 10% 1-PROPANOL AND 4% PROTEIN |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | ammonium sulfate | 3 (M) | |
| 2 | 1 | 1 | 1-propanol | 10 (%) | |
| 3 | 1 | 1 | TEL | 100mg |
