1JS9
Brome Mosaic Virus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-09-28 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.15 |
| Spacegroup name | H 3 |
| Unit cell lengths | 269.244, 269.244, 638.136 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.700 - 4.000 * |
| R-factor | 0.237 * |
| Rwork | 0.238 |
| R-free | 0.25100 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cwp |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.490 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.790 | 3.460 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.170 * | |
| Total number of observations | 2463817 * | |
| Number of reflections | 203504 | |
| <I/σ(I)> | 3.02 | 0.58 |
| Completeness [%] | 86.4 * | 55.4 |
| Redundancy | 12.1 | 1.34 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 5.2 | 18 * | PEG 550, magnesium acetate, pH 5.2, VAPOR DIFFUSION, SITTING DROP, temperature 296K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | virus | 7. (mg/ml) | |
| 2 | 1 | reservoir | PEG550 MME | 23 (%) | |
| 3 | 1 | reservoir | magnesium acetate | 0.1 (M) | pH5.2 |
