1JS9

Brome Mosaic Virus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2000-09-28
Detector	ADSC QUANTUM 4
Wavelength(s)	1.15
Spacegroup name	H 3
Unit cell lengths	269.244, 269.244, 638.136
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.700 - 4.000 *
R-factor	0.237 *
Rwork	0.238
R-free	0.25100 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1cwp
RMSD bond length	0.008
RMSD bond angle	1.490 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.790	3.460
High resolution limit [Å]	3.400	3.400
Rmerge	0.170 *
Total number of observations	2463817 *
Number of reflections	203504
<I/σ(I)>	3.02	0.58
Completeness [%]	86.4 *	55.4
Redundancy	12.1	1.34

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	5.2	18 *	PEG 550, magnesium acetate, pH 5.2, VAPOR DIFFUSION, SITTING DROP, temperature 296K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	virus	7. (mg/ml)
2	1	reservoir	PEG550 MME	23 (%)
3	1	reservoir	magnesium acetate	0.1 (M)	pH5.2