1JS8

Structure of a Functional Unit from Octopus Hemocyanin

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE
Synchrotron site	Photon Factory
Temperature [K]	279
Detector technology	IMAGE PLATE
Collection date	1989-06-01
Detector	FUJI
Wavelength(s)	1.3796,1.3779,1.2000
Spacegroup name	P 21 21 21
Unit cell lengths	92.860, 168.390, 58.290
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.300
R-factor	0.201
Rwork	0.202
R-free	0.26200
Structure solution method	MIRAS, combined with partial MAD phasing
RMSD bond length	0.014
RMSD bond angle	1.690
Data reduction software	DENZO
Data scaling software	CCP4 ((AGROVATA)
Phasing software	MADSYS
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
High resolution limit [Å]	2.300 *
Rmerge	0.066 *
Completeness [%]	94.8 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	Cuff, M.E., (1990) J. Mol. Biol., 213, 11. *
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	Cuff, M.E., (1990) J. Mol. Biol., 213, 11. *