1JS6
Crystal Structure of DOPA decarboxylase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM1A |
| Synchrotron site | ESRF |
| Beamline | BM1A |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1993-04-10 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8500 |
| Spacegroup name | P 62 |
| Unit cell lengths | 152.860, 152.860, 86.600 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.870 - 2.600 |
| R-factor | 0.206 |
| Rwork | 0.206 |
| R-free | 0.25800 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.870 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.086 * | 0.341 |
| Number of reflections | 31262 | |
| <I/σ(I)> | 9.1 | 3.5 |
| Completeness [%] | 88.4 | 67.8 |
| Redundancy | 2 | 0.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | PEG MME 5000, MES, ammonium sulfate at pH 6.5, VAPOR DIFFUSION, HANGING DROP at 298K, temperature 298.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG5000 MME | 30 (%(w/v)) | |
| 2 | 1 | reservoir | MES/KOH | 50 (mM) | |
| 3 | 1 | reservoir | ammonium sulfate | 400 (mM) | |
| 4 | 1 | drop | enzyme | 30.0 (mg/ml) |
