1JRN
Orthorhombic form of Oxytricha telomeric DNA at 2.0A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-07-10 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 26.508, 47.438, 96.464 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.000 |
| R-factor | 0.262 * |
| Rwork | 0.218 |
| R-free | 0.28200 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.014 |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 8.000 * | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.052 | 0.190 * |
| Number of reflections | 8565 | |
| <I/σ(I)> | 31.5 | 5.84 |
| Completeness [%] | 98.0 | 98.2 |
| Redundancy | 2.08 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 12 * | MPD, Potassium Chloride, Magnesium Chloride, Spermine, Cacodylate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 285K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MPD | ||
| 2 | 1 | 1 | KCL | ||
| 3 | 1 | 1 | MGCL2 | ||
| 4 | 1 | 1 | Spermine | ||
| 5 | 1 | 1 | Cacodylate |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | 10.0 (mM) | ||
| 2 | 1 | drop | 40.0 (mM) | ||
| 3 | 1 | drop | spermine | 4.10 (mM) | |
| 4 | 1 | drop | DNA | 1.0 (mM) | |
| 5 | 1 | drop | MPD | 5.0 (%(v/v)) | |
| 6 | 1 | reservoir | MPD | 35 (%(v/v)) |
