1JRN
Orthorhombic form of Oxytricha telomeric DNA at 2.0A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2001-07-10 |
Detector | RIGAKU RAXIS IV |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 26.508, 47.438, 96.464 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.000 |
R-factor | 0.262 * |
Rwork | 0.218 |
R-free | 0.28200 * |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 0.014 |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 8.000 * | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.052 | 0.190 * |
Number of reflections | 8565 | |
<I/σ(I)> | 31.5 | 5.84 |
Completeness [%] | 98.0 | 98.2 |
Redundancy | 2.08 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 12 * | MPD, Potassium Chloride, Magnesium Chloride, Spermine, Cacodylate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 285K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | MPD | ||
2 | 1 | 1 | KCL | ||
3 | 1 | 1 | MGCL2 | ||
4 | 1 | 1 | Spermine | ||
5 | 1 | 1 | Cacodylate |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | 10.0 (mM) | ||
2 | 1 | drop | 40.0 (mM) | ||
3 | 1 | drop | spermine | 4.10 (mM) | |
4 | 1 | drop | DNA | 1.0 (mM) | |
5 | 1 | drop | MPD | 5.0 (%(v/v)) | |
6 | 1 | reservoir | MPD | 35 (%(v/v)) |