1JRL
Crystal structure of E. coli Lysophospholiase L1/Acyl-CoA Thioesterase I/Protease I L109P mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL17B2 |
| Synchrotron site | NSRRC |
| Beamline | BL17B2 |
| Temperature [K] | 133 |
| Detector technology | IMAGE PLATE |
| Detector | MAC Science DIP-2030 |
| Wavelength(s) | 1.12720 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 50.011, 50.011, 169.718 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.620 - 1.950 |
| Rwork | 0.195 |
| R-free | 0.22900 |
| Structure solution method | MIR |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | XPRESS |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.620 | 1.960 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Rmerge | 0.042 | 0.384 |
| Number of reflections | 15859 | |
| <I/σ(I)> | 19.3 | 5 |
| Completeness [%] | 96.2 | 95.2 |
| Redundancy | 7.89 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 2-[N-morpholino]ethanesulfonic acid, PEGMME 5000, Ammonium sulfate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
