1JRC
The N67A mutant of Lactococcus lactis dihydroorotate dehydrogenase A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7B |
Temperature [K] | 120 |
Detector technology | IMAGE PLATE |
Collection date | 1999-04-24 |
Detector | MARRESEARCH |
Wavelength(s) | 0.84690 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 52.800, 108.090, 65.800 |
Unit cell angles | 90.00, 103.89, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.800 |
Rwork | 0.177 |
R-free | 0.22000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Lactococcus lactis DHODA PDB ID 2DOR |
RMSD bond length | 0.017 |
RMSD bond angle | 0.032 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.870 |
High resolution limit [Å] | 1.840 | 1.840 |
Rmerge | 0.059 | 0.207 |
Number of reflections | 62505 | |
<I/σ(I)> | 22.8 | 5.3 |
Completeness [%] | 98.8 | 87.2 |
Redundancy | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEG 6K, Na-acetate, TRIS-HCl, VAPOR DIFFUSION, HANGING DROP, temperature 293K |