1JR8

Crystal Structure of Erv2p

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	120
Detector technology	IMAGE PLATE
Collection date	2001-06-15
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	47.640, 45.150, 53.840
Unit cell angles	90.00, 100.15, 90.00

Refinement procedure

Resolution	50.000 - 1.500
R-factor	0.208 *
Rwork	0.208
R-free	0.23300
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.780
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.550
High resolution limit [Å]	1.500	1.500
Rmerge	0.047	0.265
Number of reflections	36002
<I/σ(I)>	32.7	6.2
Completeness [%]	99.4 *	98.2
Redundancy	3.6	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	293	used seeding *
1	VAPOR DIFFUSION, HANGING DROP	6.2	293	used seeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	cacodylic acid	0.1 (M)	pH6.2
2	1	reservoir		10 (mM)
3	1	reservoir	PEG8000	22 (%(w/v))