1JQU
Are Carboxy Terminii of Helices Coded by the Local Sequence or by Tertiary Structure Contacts
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 298 |
Detector technology | AREA DETECTOR |
Collection date | 1995-11-14 |
Detector | XUONG-HAMLIN MULTIWIRE |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 74.300, 77.200, 138.200 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.000 - 2.600 |
R-factor | 0.235 * |
Rwork | 0.229 |
R-free | 0.31500 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | T4 Lysozyme 2LZM |
RMSD bond length | 0.022 * |
RMSD bond angle | 2.200 * |
Phasing software | CNS |
Refinement software | TNT |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.900 | 2.700 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.057 * | |
Number of reflections | 22095 | |
<I/σ(I)> | 6.9 | 1.2 |
Completeness [%] | 78.0 * | 71.5 |
Redundancy | 3.42 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7 * | 277 | 30% PEG4000, PIPES buffer ph7.0, 0.2M LiSO4, pH 6.75, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 15 (mg/ml) | |
2 | 1 | drop | HEPES | 20 (mM) | pH7.0 |
3 | 1 | reservoir | PEG4000 | 30 (%) | |
4 | 1 | reservoir | PIPES | 0.1 (M) | pH7.0 |
5 | 1 | reservoir | 0.2 (M) |