1JQL
Mechanism of Processivity Clamp Opening by the Delta Subunit Wrench of the Clamp Loader Complex of E. coli DNA Polymerase III: Structure of beta-delta (1-140)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2000-06-05 |
| Detector | CUSTOM-MADE |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 110.100, 110.100, 134.900 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 500.000 - 2.500 |
| R-factor | 0.249 * |
| Rwork | 0.249 |
| R-free | 0.29400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Model of the beta-delta complex of DNA Polymerase III |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.628 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 500.000 |
| High resolution limit [Å] | 2.500 |
| Rmerge | 0.080 * |
| Total number of observations | 740503 * |
| Number of reflections | 36450 |
| Completeness [%] | 98.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 8 | 294 | TRIS-HCL, polyethylene glycol 4000, MgCl2, dithiothreitol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 21K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 30 (mg/ml) | |
| 2 | 1 | 1 | Tris-HCl | 50 (mM) | |
| 3 | 1 | 1 | PEG4000 | 10-11 (%) | |
| 4 | 1 | 1 | 100 (mM) | ||
| 5 | 1 | 1 | dithiothreitol | 2 (mM) |
