1JQG
Crystal Structure of the Carboxypeptidase A from Helicoverpa Armigera
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-01-16 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.030, 86.390, 50.550 |
| Unit cell angles | 90.00, 100.70, 90.00 |
Refinement procedure
| Resolution | 12.000 * - 2.500 |
| R-factor | 0.18 |
| Rwork | 0.180 |
| R-free | 0.23810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1aye |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.500 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 12.000 * | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.092 | 0.344 * |
| Total number of observations | 230224 * | |
| Number of reflections | 21449 | |
| <I/σ(I)> | 15.6 | 2.5 |
| Completeness [%] | 93.2 | 93 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 8 * | 291 | used macroseeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | Tris-HCl | 5 (mM) | pH8.0 |
| 2 | 1 | 1 | 0.001 (mM) | ||
| 3 | 1 | 1 | protein | 10 (mg/ml) | |
| 4 | 1 | 2 | PEG8000 | 30 (%(w/v)) | |
| 5 | 1 | 2 | sodium acetate | 0.2 (M) | |
| 6 | 1 | 2 | sodium cacodylate | 0.1 (M) | pH5.5 |
