1JQF

Human Transferrin N-Lobe Mutant H249Q

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	2000-10-01
Detector	MARRESEARCH
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	44.200, 57.200, 135.750
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.850
R-factor	0.221 *
Rwork	0.221
R-free	0.24600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1a8e
RMSD bond length	0.005
RMSD bond angle	1.250
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.920
High resolution limit [Å]	1.850	1.850
Rmerge	0.087	0.394
Number of reflections	30081 *
<I/σ(I)>	17	2.9
Completeness [%]	99.4	96.5
Redundancy	4.6	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	291	used microseeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	35 (mg/ml)
2	1	reservoir	potassium acetate	0.1 (M)
3	1	reservoir	PEG3350	20-25 (%)