1JQ5
Bacillus Stearothermophilus Glycerol dehydrogenase complex with NAD+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.2 |
| Synchrotron site | SRS |
| Beamline | PX14.2 |
| Temperature [K] | 290 |
| Detector technology | CCD |
| Collection date | 1999-11-29 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.979 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 104.660, 104.660, 148.510 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.700 |
| R-factor | 0.151 * |
| Rwork | 0.151 |
| R-free | 0.19030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jpu |
| RMSD bond length | 0.013 |
| RMSD bond angle | 21.267 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 1.740 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.047 | 0.376 |
| Total number of observations | 196167 * | |
| Number of reflections | 42016 | |
| <I/σ(I)> | 19.9 | 2.7 |
| Completeness [%] | 92.1 | 78.6 |
| Redundancy | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 290 | Burke, J., (2001) Acta Crystallogr., Sect.D, 57, 165. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | reservoir | ammonium sulfate | 0.5-2.0 (M) | |
| 3 | 1 | reservoir | PEG400 | 2-6 (%) | |
| 4 | 1 | reservoir | 0.020 (mM) |
