1JPX

Mutation that destabilize the gp41 core: determinants for stabilizing the SIV/CPmac envelope glycoprotein complex. Wild type.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	95
Detector technology	CCD
Collection date	2001-04-15
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9480
Spacegroup name	P 63
Unit cell lengths	54.993, 54.993, 131.140
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 * - 2.300
R-factor	0.237 *
Rwork	0.237
R-free	0.28300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qbz
RMSD bond length	0.007
RMSD bond angle	17.700 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.057	0.209
Total number of observations	129420 *
Number of reflections	9729
<I/σ(I)>	11.7	8.1
Completeness [%]	97.2	94
Redundancy	6.7	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	Sodium chloride, CTAB, barium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir		0.6 (M)
3	1	reservoir		0.04 (M)
4	1	reservoir	hexadecyltrimethylammonium bromide	0.01 (M)