1JPS
Crystal structure of tissue factor in complex with humanized Fab D3h44
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-05-18 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8428 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 84.250, 93.280, 110.010 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.850 |
| R-factor | 0.201 * |
| Rwork | 0.201 |
| R-free | 0.22500 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ahw |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.640 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.960 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Number of reflections | 73056 | |
| <I/σ(I)> | 12.6 | 3.7 |
| Completeness [%] | 96.8 | 94.4 |
| Redundancy | 3.4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.5 | 4 * | PEG 6000, sodium chloride, sodium-HEPES buffer, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 12 (mg/ml) | |
| 2 | 1 | drop | HEPES | 20 (mM) | pH7.5 |
| 3 | 1 | drop | 0.01 (%(w/v)) | ||
| 4 | 1 | reservoir | PEG6000 | 11 (%) | |
| 5 | 1 | reservoir | 2.0 (M) | ||
| 6 | 1 | reservoir | HEPES | 0.1 (M) | pH7.5 |
