1JPQ
Crystal Structure of the Oxytricha Telomeric DNA at 1.6A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-11-02 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.98 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 27.543, 27.543, 145.817 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 99.000 - 1.600 |
R-factor | 0.229 * |
Rwork | 0.225 |
R-free | 0.27500 * |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.019 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 8.000 * | 1.660 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.094 | 0.220 * |
Number of reflections | 8862 | |
<I/σ(I)> | 18.6 | 6.38 |
Completeness [%] | 96.8 | 98.4 |
Redundancy | 1.12 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 285 | potassium chloride, magnesium chloride, spermine, MPD, potassium cacodylate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 12K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | KCl | ||
2 | 1 | 1 | MgCl2 | ||
3 | 1 | 1 | spermine | ||
4 | 1 | 1 | MPD | ||
5 | 1 | 1 | potassium cacodylate |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | 10.0 (mM) | ||
2 | 1 | drop | 40.0 (mM) | ||
3 | 1 | drop | 3.3 (mM) | ||
4 | 1 | drop | DNA | 1.0 (mM) | |
5 | 1 | drop | MPD | 5.0 (%(v/v)) | |
6 | 1 | reservoir | MPD | 25 (%(v/v)) |