1JPL
GGA3 VHS domain complexed with C-terminal peptide from cation-independent mannose 6-phosphate receptor
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9B |
| Synchrotron site | NSLS |
| Beamline | X9B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-06-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97900, 0.97938, 0.95369 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 108.300, 125.300, 64.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.000 * - 2.400 |
| R-factor | 0.214 * |
| Rwork | 0.213 |
| R-free | 0.25800 * |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.078 * | 0.303 |
| Number of reflections | 34790 | 3381 * |
| <I/σ(I)> | 26.7 | 6.7 |
| Completeness [%] | 99.5 | 99.3 |
| Redundancy | 10.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 10.6 | 293 | 1.5M Sodium/Potassium Phosphate, 200mM Lithium Sulfate, pH 10.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 12 (mg/ml) | |
| 2 | 1 | drop | peptide | 1.5 (mM) | |
| 3 | 1 | reservoir | CAPS | 100 (mM) | pH10.2-11.0 |
| 4 | 1 | reservoir | 200 (mM) | ||
| 5 | 1 | reservoir | sodium potassium phospahte | 1.33-2 (M) |
