1JPJ

GMPPNP Complex of SRP GTPase NG Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 5ID-B
Synchrotron site	APS
Beamline	5ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	1998-11-15
Detector	MARRESEARCH
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	52.690, 60.410, 86.060
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.300
R-factor	0.203 *
Rwork	0.201
R-free	0.29900 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ffh
RMSD bond length	0.004
RMSD bond angle	24.570 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.064	0.171
Number of reflections	10955
Completeness [%]	85.3	95.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	8	4 *	10% PEG 1000, 10% PEG 6000, pH 8, VAPOR DIFFUSION, SITTING DROP, temperature 277.15K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20-30 (mg/ml)
2	1	drop		2 (mM)
3	1	reservoir	PEG1000	10 (%(w/v))
4	1	reservoir	PEG6000	10 (%(w/v))