1JPF
Crystal Structure Of The LCMV Peptidic Epitope Gp276 In Complex With The Murine Class I Mhc Molecule H-2Db
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM1A |
| Synchrotron site | ESRF |
| Beamline | BM1A |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-02-28 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.873 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 56.166, 56.166, 277.954 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.180 |
| R-factor | 0.211 * |
| Rwork | 0.211 |
| R-free | 0.26200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 * |
| RMSD bond angle | 1.490 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.230 |
| High resolution limit [Å] | 2.180 | 2.180 |
| Rmerge | 0.082 * | 0.293 * |
| Number of reflections | 24648 | 1569 * |
| Completeness [%] | 99.8 | 98.3 |
| Redundancy | 8.6 * | 6.9 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 4 * | Ammonium phosphate, PEG8000, Tris-HCl, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10.6 (mg/ml) | |
| 2 | 1 | reservoir | ammonium phosphate | 0.2 (M) | |
| 3 | 1 | reservoir | PEG8000 | 25 (%) | |
| 4 | 1 | reservoir | Tris-HCl | 0.1 (M) | |
| 5 | 1 | reservoir | sodium azide | 0.02 (%(w/v)) |
