1JPD
L-Ala-D/L-Glu Epimerase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 113 |
| Detector technology | CCD |
| Collection date | 1999-12-01 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.70008 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 84.203, 84.203, 158.881 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.400 - 2.600 |
| R-factor | 0.209 |
| Rwork | 0.209 |
| R-free | 0.24500 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.053 | 0.286 |
| Total number of observations | 101261 * | |
| Number of reflections | 17379 * | |
| <I/σ(I)> | 22.1 | 9.1 |
| Completeness [%] | 95.3 | 97 |
| Redundancy | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 400-500mM NaCl, 50 mM Succinate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG400 | 8 (%) | |
| 2 | 1 | reservoir | 0.2 (M) | ||
| 3 | 1 | reservoir | Bis-Tris | 50 (mM) | pH5.0 |
