1JP9
Sperm Whale met-Myoglobin (low temperature; high pressure)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2000-02-02 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9503 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.079, 30.777, 34.404 |
| Unit cell angles | 90.00, 105.42, 90.00 |
Refinement procedure
| Resolution | 26.000 * - 1.700 |
| R-factor | 0.21 * |
| Rwork | 0.210 |
| R-free | 0.24700 |
| Starting model (for MR) | 1vxh |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.975 * |
| Data reduction software | DPS |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.000 | 1.810 |
| High resolution limit [Å] | 1.700 | 1.670 |
| Rmerge | 0.075 | 0.096 |
| Number of reflections | 14428 | |
| <I/σ(I)> | 4.8 | 6.8 |
| Completeness [%] | 98.5 | 97.7 |
| Redundancy | 4 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 6 | 298 | ammonium sulfate, pH 6, batch, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | ammonium sulfate | 72-78 (%sat) | pH5.5-6.0 |
