1JOW
Crystal structure of a complex of human CDK6 and a viral cyclin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-12-17 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.99999 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 70.140, 70.140, 448.770 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 * - 3.100 |
R-factor | 0.266 * |
Rwork | 0.266 |
R-free | 0.32300 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.500 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.190 |
High resolution limit [Å] | 3.100 | 3.100 |
Rmerge | 0.058 * | 0.290 * |
Total number of observations | 92567 * | |
Number of reflections | 12678 * | |
<I/σ(I)> | 29 | 4 |
Completeness [%] | 97.9 * | 81.8 * |
Redundancy | 7.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | Schulze-Gahmen, U., (2001) Acta Crystallogr, D57, 1287. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 25 (mM) | pH8.0 |
3 | 1 | drop | 150 (mM) | ||
4 | 1 | drop | dithiothreitol | 10 (mM) | |
5 | 1 | drop | EDTA | 0.5 (mM) | |
6 | 1 | reservoir | calcium acetate | 0.1 (M) | |
7 | 1 | reservoir | PEG3350 | 10 (%) | |
8 | 1 | reservoir | 0.15 (M) |