1JOS

Ribosome Binding Factor A(rbfA)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	115
Detector technology	CCD
Collection date	2000-06-01
Detector	BRUKER
Wavelength(s)	1.0113
Spacegroup name	P 21 21 21
Unit cell lengths	46.230, 48.090, 57.960
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 1.700
Rwork	0.216
R-free	0.28300
Structure solution method	MAD
RMSD bond length	0.016
RMSD bond angle	1.600
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	1.740
High resolution limit [Å]	1.700	1.700
Rmerge	0.124
Number of reflections	10622
<I/σ(I)>	12.7	3.5
Completeness [%]	72.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.3	293	0.6M NaK tartrate, 10 mM HEPES, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K