1JON
GROEL (HSP60 CLASS) FRAGMENT COMPRISING RESIDUES 191-345
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-04-09 |
| Detector | MARRESEARCH |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 91.670, 91.670, 38.330 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 2.500 |
| R-factor | 0.214 * |
| Rwork | 0.216 |
| R-free | 0.29100 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oel |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.423 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 |
| Phasing software | AMoRE |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.000 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.099 | 0.451 |
| Total number of observations | 21762 * | |
| Number of reflections | 6564 | |
| <I/σ(I)> | 9.9 | 1.8 |
| Completeness [%] | 99.4 | 96.7 |
| Redundancy | 3.3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8.5 | 290 | 11% PEG 4000, 50 MM TRIS-HCL, PH 8.5, 200 MM LISO4, 23 MG/ML PROTEIN, 17 DEG. C., temperature 290K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 23 (mg/ml) | |
| 2 | 1 | drop | PEG4000 | 11 (%) | |
| 3 | 1 | drop | Tris-HCl | 50 (mM) | |
| 4 | 1 | drop | 100 (mM) | ||
| 5 | 1 | reservoir | PEG4000 | 22 (%) | |
| 6 | 1 | reservoir | Tris-HCl | 100 (mM) | |
| 7 | 1 | reservoir | 200 (mM) |
