1JOI
STRUCTURE OF PSEUDOMONAS FLUORESCENS HOLO AZURIN
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 300 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 1992-02 |
| Detector | ENRAF-NONIUS FAST |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 31.950, 43.780, 78.810 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.050 |
| R-factor | 0.17 |
| Rwork | 0.170 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2aza |
| RMSD bond length | 0.010 |
| RMSD bond angle | 2.590 |
| Data reduction software | CCP4 |
| Data scaling software | CCP4 |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.140 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.051 | |
| Number of reflections | 7076 | |
| <I/σ(I)> | 7.84 | |
| Completeness [%] | 80.02 | |
| Redundancy | 2.45 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | CONVENTIONAL VAPOR DIFFUSION OF HANGING DROP. 3M OF AMMONIUM SULFATE BUFFERED WITH 0.2 TRIS BUFFER AT PH7.5 IN RESERVOIR. DROPS MADE OF 6 MICROLITERS OF PROTEIN SOLUTION (6.2 MG/ML) AND EQUAL VOLUME OF RESERVOIR SOLUTION., vapor diffusion - hanging drop |
