1JOC
EEA1 homodimer of C-terminal FYVE domain bound to inositol 1,3-diphosphate
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12C |
Synchrotron site | NSLS |
Beamline | X12C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-03-20 |
Detector | BRANDEIS - B1 |
Wavelength(s) | 1.28322, 1.28356, 1.23985 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 36.528, 85.096, 88.036 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.200 |
R-factor | 0.221 * |
Rwork | 0.221 |
R-free | 0.28100 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.100 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | X-PLOR (3.1) |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 4 * | 11% PEG 4000, 50 mM HEPES, 60 mM ammonium acetate, 10% glycerol, and 1.5 mM Ins(1,3)P2, pH 7.0, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 12 (mg/ml) | |
2 | 1 | reservoir | PEG4000 | 11 (%) | |
3 | 1 | reservoir | HEPES | 50 (mM) | pH7.0 |
4 | 1 | reservoir | ammonium acetate | 60 (mM) | |
5 | 1 | reservoir | Ins(1,3)P2 | 1.5 (mM) |