1JO8
Structural analysis of the yeast actin binding protein Abp1 SH3 domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2000-09-15 |
Detector | MARRESEARCH |
Wavelength(s) | 0.842 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 24.080, 38.155, 59.090 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 17.510 - 1.300 |
R-factor | 0.14512 |
Rwork | 0.144 |
R-free | 0.17785 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1cka |
RMSD bond length | 0.011 |
RMSD bond angle | 1.714 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 * | 1.320 |
High resolution limit [Å] | 1.300 | 1.300 |
Rmerge | 0.056 | 0.266 |
Total number of observations | 56424 * | |
Number of reflections | 13742 * | |
<I/σ(I)> | 20.3 | 4 |
Completeness [%] | 98.0 | 88.6 |
Redundancy | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | Ammonium sulfate, bis-tris-propane, pH 8.0, VAPOR DIFFUSION, SITTING DROP at 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | ammonium sulfate | 3.2 (M) | |
2 | 1 | reservoir | Bis-Tris propane | 100 (mM) | pH8.0 |
3 | 1 | drop | Abp1-SH3 | 12 (mg/ml) |