1JO2
Crystal Structure of the B-DNA Hexamer (CgATCG).Daunomycin Complex Containing a Ribose at the Intercalation Site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-06-24 |
| Detector | RIGAKU RAXIS IIC |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 28.050, 28.050, 53.160 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.500 |
| Rwork | 0.198 |
| R-free | 0.23300 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 17.500 * |
| Data scaling software | SCALEPACK |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 10.000 |
| High resolution limit [Å] | 1.500 |
| Number of reflections | 2767 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 | 293 | magnesium chloride, cacodylate, MPD, pH 7.00, EVAPORATION, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MgCl2 | ||
| 2 | 1 | 1 | cacodylate | ||
| 3 | 1 | 1 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | oligonucleotide | 2 (mM) | single strand concentration |
| 2 | 1 | drop | sodium cacodylate | 40 (mM) | pH7.0 |
| 3 | 1 | drop | 24 (mM) | ||
| 4 | 1 | drop | daunomycin | 2 (mM) | |
| 5 | 1 | drop | MPD | 2 (%(v/v)) | |
| 6 | 1 | reservoir | MPD | 60 (%) |
