1JO0
Structure of HI1333, a Hypothetical Protein from Haemophilus influenzae with Structural Similarity to RNA-binding Proteins
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-BM |
| Synchrotron site | APS |
| Beamline | 17-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-03-10 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.91840 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 30.566, 51.941, 59.100 |
| Unit cell angles | 90.00, 102.19, 90.00 |
Refinement procedure
| Resolution | 25.900 - 1.370 |
| Rwork | 0.138 |
| R-free | 0.22100 * |
| Structure solution method | MAD (PT & BR) |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.030 |
| Data reduction software | XPREP (6.09) |
| Data scaling software | SCALEPACK (1.96.1) |
| Phasing software | SOLVE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.900 | 1.400 |
| High resolution limit [Å] | 1.370 | 1.370 |
| Rmerge | 0.031 | 0.271 |
| Total number of observations | 246633 * | |
| Number of reflections | 38069 * | |
| <I/σ(I)> | 26.79 | 3.06 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.47 | 3.63 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microdialysis * | 7 * | 298 | 28-30% PEG4000, 100mM Tris pH 8.0, 10mM CaCl2, + 1.5% heptane-1,2,3-triol added to drop, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | sodium phosphate | 20 (mM) | pH7.0 |
| 2 | 1 | 1 | 100 (mM) | ||
| 3 | 1 | 2 | protein | 18 (mg/ml) |
