1JO0

Structure of HI1333, a Hypothetical Protein from Haemophilus influenzae with Structural Similarity to RNA-binding Proteins

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-BM
Synchrotron site	APS
Beamline	17-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2001-03-10
Detector	MARRESEARCH
Wavelength(s)	0.91840
Spacegroup name	P 1 21 1
Unit cell lengths	30.566, 51.941, 59.100
Unit cell angles	90.00, 102.19, 90.00

Refinement procedure

Resolution	25.900 - 1.370
Rwork	0.138
R-free	0.22100 *
Structure solution method	MAD (PT & BR)
RMSD bond length	0.011
RMSD bond angle	0.030
Data reduction software	XPREP (6.09)
Data scaling software	SCALEPACK (1.96.1)
Phasing software	SOLVE
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.900	1.400
High resolution limit [Å]	1.370	1.370
Rmerge	0.031	0.271
Total number of observations	246633 *
Number of reflections	38069 *
<I/σ(I)>	26.79	3.06
Completeness [%]	99.9	100
Redundancy	6.47	3.63

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	7 *	298	28-30% PEG4000, 100mM Tris pH 8.0, 10mM CaCl2, + 1.5% heptane-1,2,3-triol added to drop, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	sodium phosphate	20 (mM)	pH7.0
2	1	1		100 (mM)
3	1	2	protein	18 (mg/ml)