1JNZ
Structure of adenylylsulfate reductase from the hyperthermophilic Archaeoglobus fulgidus at 1.6 resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
Synchrotron site | MPG/DESY, HAMBURG |
Beamline | BW6 |
Temperature [K] | 70 |
Detector technology | CCD |
Collection date | 1999-11-29 |
Detector | MARRESEARCH |
Wavelength(s) | 1.06 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 72.600, 113.500, 193.900 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.190 - 2.500 |
R-factor | 0.161 |
Rwork | 0.161 |
R-free | 0.19900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1jnr |
RMSD bond length | 0.008 |
RMSD bond angle | 1.400 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.530 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 53527 | |
Completeness [%] | 79.2 | 71.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 277 | PEG 4000, sodium acetate, sodium chloride, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |