1JNY
Crystal structure of Sulfolobus solfataricus elongation factor 1 alpha in complex with GDP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ELETTRA BEAMLINE 5.2R |
Synchrotron site | ELETTRA |
Beamline | 5.2R |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 1998-09-06 |
Detector | MARRESEARCH |
Wavelength(s) | 1.000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 62.113, 113.718, 80.320 |
Unit cell angles | 90.00, 90.20, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.800 |
R-factor | 0.224 |
Rwork | 0.220 |
R-free | 0.26900 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 24.200 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.850 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.034 | 0.172 |
Total number of observations | 762914 * | |
Number of reflections | 101657 | |
<I/σ(I)> | 38.7 | 8.7 |
Completeness [%] | 98.6 | 95.5 |
Redundancy | 7.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH UNDER OIL | 5.6 | 277 | Zagari, A., (1994) J. Mol. Biol., 242, 175. * |