1JNX
Crystal structure of the BRCT repeat region from the breast cancer associated protein, BRCA1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-02-04 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 114.844, 114.844, 122.111 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 2.500 |
| R-factor | 0.262 |
| Rwork | 0.259 |
| R-free | 0.30200 |
| Structure solution method | MAD |
| RMSD bond length | 0.017 * |
| RMSD bond angle | 1.810 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.570 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.048 * | 0.292 * |
| Total number of observations | 209566 * | |
| Number of reflections | 16968 | |
| Completeness [%] | 99.8 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 20-23 * | Lithium sulphate, Nickel sulphate, calcium chloride, Tris, pH 8.5,VAPOR DIFFUSION, HANGING DROP at 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 18 (mg/ml) | |
| 2 | 1 | reservoir | 0.8 (M) | ||
| 3 | 1 | reservoir | Tris-HCl | 100 (mM) | pH8.5 |
| 4 | 1 | reservoir | 2.5 (mM) | ||
| 5 | 1 | reservoir | 5 (mM) |
