1JNU
Photoexcited structure of the plant photoreceptor domain, phy3 LOV2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 296 |
| Detector technology | CCD |
| Collection date | 2001-06-05 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 44.843, 54.771, 70.963 |
| Unit cell angles | 93.14, 93.79, 90.40 |
Refinement procedure
| Resolution | 30.000 * - 2.600 |
| R-factor | 0.238 * |
| Rwork | 0.236 |
| R-free | 0.25900 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | phy3 LOV2 dark-state structure |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.360 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 * | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.044 | 0.190 |
| Number of reflections | 17700 | |
| <I/σ(I)> | 12.3 | 3.2 |
| Completeness [%] | 87.5 | 79 |
| Redundancy | 1.6 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 8 | 25 * | PEG 5000 MME, Tris, Glycerol, pH 8.0, VAPOR DIFFUSION, HANGING DROP at 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | PEG5000 MME | 22 (%) | |
| 2 | 1 | 1 | glycerol | 12 (%) | |
| 3 | 1 | 1 | Tris | 50 (mM) | pH8.0 |
| 4 | 1 | 2 | protein | 15 (mg/ml) |
