1JNP

Crystal Structure of Murine Tcl1 at 2.5 Resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12B
Synchrotron site	NSLS
Beamline	X12B
Temperature [K]	90
Detector technology	CCD
Collection date	1999-10-11
Detector	ADSC QUANTUM 4
Spacegroup name	C 1 2 1
Unit cell lengths	89.323, 115.911, 37.945
Unit cell angles	90.00, 115.15, 90.00

Refinement procedure

Resolution	10.000 - 2.500
R-factor	0.2257
Rwork	0.226
R-free	0.23560
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.019 *
RMSD bond angle	2.100 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000 *	2.540
High resolution limit [Å]	2.500	2.500
Rmerge	0.059 *
Total number of observations	11802 *
Number of reflections	11019 *
<I/σ(I)>	11.6
Completeness [%]	99.8 *	99.5 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.5 *	293	Sodium Acetate, Tris, 1-O-octyl-beta-D-glucopyranoside, pH 3.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
2	1	drop	Tris	10 (mM)
3	1	reservoir	sodium acetate	0.05 (M)
4	1	reservoir	Tris	0.1 (M)