1JNF
Rabbit serum transferrin at 2.6 A resolution.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 1993-12-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.87 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 127.190, 127.190, 144.850 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.600 |
| R-factor | 0.187 |
| Rwork | 0.185 |
| R-free | 0.22300 |
| Starting model (for MR) | Structure at 3.3 A determined by MIR |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.942 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((AGROVATA) |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.670 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.057 | 0.248 |
| Number of reflections | 36323 | |
| <I/σ(I)> | 9.9 | 2.7 |
| Completeness [%] | 97.7 | 85.6 |
| Redundancy | 5.6 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch crystallisation | 5.4 | 279 | 0.01M Tris/HCl, acetic acid or HCl, pH 5.4, Batch crystallisation, temperature 279K |
