1JNE
Crystal structure of imaginal disc growth factor-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-05-25 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.927 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.520, 72.170, 105.780 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.700 |
| R-factor | 0.195 |
| Rwork | 0.195 |
| R-free | 0.25900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ctn |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.540 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.740 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.043 * | 0.274 * |
| Total number of observations | 303904 * | |
| Number of reflections | 40966 | |
| <I/σ(I)> | 6.05 | |
| Completeness [%] | 98.2 | 80.8 |
| Redundancy | 1 | 2.84 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7 | 25 * | PEG 4000, Tris, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 2 | PEG4000 | 25 (%) | |
| 2 | 1 | 2 | Tris | 0.1 (M) | pH7.0 |
| 3 | 1 | 1 | protein | 15 (mg/ml) |
