1JMA

CRYSTAL STRUCTURE OF THE HERPES SIMPLEX VIRUS GLYCOPROTEIN D BOUND TO THE CELLULAR RECEPTOR HVEA/HVEM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Collection date	2000-05-19
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9196
Spacegroup name	P 31 2 1
Unit cell lengths	129.154, 129.154, 80.940
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.650
R-factor	0.236 *
Rwork	0.236
R-free	0.25900
Structure solution method	MIRAS
RMSD bond length	0.008
RMSD bond angle	1.546
Data reduction software	DENZO
Data scaling software	CCP4 ((TRUNCATE))
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	20.000
High resolution limit [Å]	2.650	2.650
Rmerge	0.068	0.346
Total number of observations	272205 *
Number of reflections	24260
<I/σ(I)>	11	5
Completeness [%]	99.7	99.9 *
Redundancy	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	8	291	1.8M Ammonium Sulfate and 5% Peg400 and Tris-HCL 100mM pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	Tris-HCl	50 (mM)
2	1	1	ammonium sulfate	1.8 (M)
3	1	1	PEG400	5 (%)