Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-05-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.380, 80.120, 99.930 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 193.800 * - 1.700 |
| R-factor | 0.197 * |
| Rwork | 0.197 |
| R-free | 0.24800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | THEORETICAL MODEL |
| RMSD bond length | 0.030 |
| RMSD bond angle | 2.220 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.100 | 1.800 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.096 | 0.409 |
| Total number of observations | 177455 * | |
| Number of reflections | 33538 | |
| <I/σ(I)> | 14 | 2.8 |
| Completeness [%] | 99.4 | 96.6 |
| Redundancy | 5.3 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | PEG 400 , Mn(CH3COO)2 , DMSO, Tris-HCl, pH 7.50, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 100 (mg/ml) | |
| 2 | 1 | reservoir | PEG400 | ||
| 3 | 1 | reservoir | 0.03 (M) | ||
| 4 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH7.5 |
