1JLX

AGGLUTININ IN COMPLEX WITH T-DISACCHARIDE

Experimental procedure

Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	273
Detector technology	CCD
Collection date	1994-08-07
Spacegroup name	P 21 21 21
Unit cell lengths	111.300, 99.200, 66.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.200
R-factor	0.196
Rwork	0.196
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1jly
RMSD bond length	0.011
RMSD bond angle	26.970 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]		2.200
Rmerge	0.109	0.272
Number of reflections	33071
<I/σ(I)>	11.3	7.4
Completeness [%]	88.4
Redundancy	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.7		pH 6.7

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	7.5 (mg/ml)
2	1	drop	sodium azide	0.01 (%)
3	1	drop	HEPES	55 (mM)
4	1	drop	PEG400	15 (%)
5	1	reservoir	PEG400	30 (%)
6	1	reservoir	HEPES	100 (mM)