1JLX
AGGLUTININ IN COMPLEX WITH T-DISACCHARIDE
Experimental procedure
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 273 |
Detector technology | CCD |
Collection date | 1994-08-07 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 111.300, 99.200, 66.300 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.200 |
R-factor | 0.196 |
Rwork | 0.196 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1jly |
RMSD bond length | 0.011 |
RMSD bond angle | 26.970 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | Outer shell | |
High resolution limit [Å] | 2.200 | |
Rmerge | 0.109 | 0.272 |
Number of reflections | 33071 | |
<I/σ(I)> | 11.3 | 7.4 |
Completeness [%] | 88.4 | |
Redundancy | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6.7 | pH 6.7 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 7.5 (mg/ml) | |
2 | 1 | drop | sodium azide | 0.01 (%) | |
3 | 1 | drop | HEPES | 55 (mM) | |
4 | 1 | drop | PEG400 | 15 (%) | |
5 | 1 | reservoir | PEG400 | 30 (%) | |
6 | 1 | reservoir | HEPES | 100 (mM) |