1JLW

Anopheles dirus species B glutathione S-transferases 1-4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	AREA DETECTOR
Collection date	2000-04-12
Detector	MARRESEARCH
Wavelength(s)	1.5405
Spacegroup name	P 32 2 1
Unit cell lengths	49.440, 49.440, 271.810
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.920 - 2.450
R-factor	0.223
Rwork	0.223
R-free	0.27100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Lucilia cuprina GST
RMSD bond length	0.007
RMSD bond angle	21.400 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.540
High resolution limit [Å]	2.450	2.450
Number of reflections	14237
<I/σ(I)>	13.1	2.2
Completeness [%]	93.9	83.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5 *	298	PEG 8000, glutathione, cacodylate, glycerol, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	sodium acetate	0.2 (M)
2	1	reservoir	PEG8000	30 (%)
3	1	reservoir	sodium cacodylate	0.1 (M)	pH6.5