1JLT

Vipoxin Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-01-01
Detector	MARRESEARCH
Wavelength(s)	0.9080
Spacegroup name	P 21 21 21
Unit cell lengths	44.049, 54.708, 104.760
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.400
R-factor	0.182 *
Rwork	0.182
R-free	0.19500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1aok
RMSD bond length	0.009
RMSD bond angle	22.700 *
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.390
High resolution limit [Å]	1.370	1.370
Rmerge	0.067	0.257
Number of reflections	53423
<I/σ(I)>	23	4
Completeness [%]	99.1	96.1
Redundancy	4.3	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.8	287 *	PEG 4000, MPD, pH 4.8, VAPOR DIFFUSION, HANGING DROP

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	PEG4000	5 (%(v/v))
3	1	drop	MPD	18 (%(v/v))