1JLR
STRUCTURE OF THE URACIL PHOSPHORIBOSYLTRANSFERASE GTP COMPLEX 2 MUTANT C128V
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 1994-03-23 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 60.600, 141.800, 71.400 |
Unit cell angles | 90.00, 115.00, 90.00 |
Refinement procedure
Resolution | 16.140 - 2.450 |
R-factor | 0.184 * |
Rwork | 0.184 |
R-free | 0.24200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1bd3 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.500 |
Data reduction software | bioteX |
Data scaling software | bioteX |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 16.100 | 2.600 |
High resolution limit [Å] | 2.450 | 2.400 * |
Rmerge | 0.039 | 0.143 |
Total number of observations | 77473 * | |
Number of reflections | 34646 | |
<I/σ(I)> | 10.6 | 3.8 |
Completeness [%] | 86.6 | 67 |
Redundancy | 3 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.7 * | 278 | Citrate buffer, NaCl, Ammonium phosphate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 278K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | 0.2 (M) | ||
2 | 1 | reservoir | sodium citrate/phosphate | 15 (mM) | pH4.7 |
3 | 1 | reservoir | PEG3400 | 10 (%) |