1JLR

STRUCTURE OF THE URACIL PHOSPHORIBOSYLTRANSFERASE GTP COMPLEX 2 MUTANT C128V

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1994-03-23
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	60.600, 141.800, 71.400
Unit cell angles	90.00, 115.00, 90.00

Refinement procedure

Resolution	16.140 - 2.450
R-factor	0.184 *
Rwork	0.184
R-free	0.24200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bd3
RMSD bond length	0.007
RMSD bond angle	1.500
Data reduction software	bioteX
Data scaling software	bioteX
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	16.100	2.600
High resolution limit [Å]	2.450	2.400 *
Rmerge	0.039	0.143
Total number of observations	77473 *
Number of reflections	34646
<I/σ(I)>	10.6	3.8
Completeness [%]	86.6	67
Redundancy	3	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.7 *	278	Citrate buffer, NaCl, Ammonium phosphate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 278K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir		0.2 (M)
2	1	reservoir	sodium citrate/phosphate	15 (mM)	pH4.7
3	1	reservoir	PEG3400	10 (%)