1JLR
STRUCTURE OF THE URACIL PHOSPHORIBOSYLTRANSFERASE GTP COMPLEX 2 MUTANT C128V
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-03-23 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.600, 141.800, 71.400 |
| Unit cell angles | 90.00, 115.00, 90.00 |
Refinement procedure
| Resolution | 16.140 - 2.450 |
| R-factor | 0.184 * |
| Rwork | 0.184 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bd3 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.500 |
| Data reduction software | bioteX |
| Data scaling software | bioteX |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 16.100 | 2.600 |
| High resolution limit [Å] | 2.450 | 2.400 * |
| Rmerge | 0.039 | 0.143 |
| Total number of observations | 77473 * | |
| Number of reflections | 34646 | |
| <I/σ(I)> | 10.6 | 3.8 |
| Completeness [%] | 86.6 | 67 |
| Redundancy | 3 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.7 * | 278 | Citrate buffer, NaCl, Ammonium phosphate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 278K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | 0.2 (M) | ||
| 2 | 1 | reservoir | sodium citrate/phosphate | 15 (mM) | pH4.7 |
| 3 | 1 | reservoir | PEG3400 | 10 (%) |
