1JLK

Crystal structure of the Mn(2+)-bound form of response regulator Rcp1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	2000-01-01
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.54
Spacegroup name	P 1 21 1
Unit cell lengths	40.511, 78.561, 43.710
Unit cell angles	90.00, 107.68, 90.00

Refinement procedure

Resolution	14.700 - 2.300
Rwork	0.225
R-free	0.28500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1i3c
RMSD bond length	0.008
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.340
High resolution limit [Å]	2.300	2.300
Rmerge	0.047	0.096
Number of reflections	10745
<I/σ(I)>	29.1	12.6
Completeness [%]	92.0	78.8
Redundancy	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	294	Ammonium sulfate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K