1JLK
Crystal structure of the Mn(2+)-bound form of response regulator Rcp1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 110 |
Detector technology | IMAGE PLATE |
Collection date | 2000-01-01 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.54 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 40.511, 78.561, 43.710 |
Unit cell angles | 90.00, 107.68, 90.00 |
Refinement procedure
Resolution | 14.700 - 2.300 |
Rwork | 0.225 |
R-free | 0.28500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1i3c |
RMSD bond length | 0.008 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 2.340 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.047 | 0.096 |
Number of reflections | 10745 | |
<I/σ(I)> | 29.1 | 12.6 |
Completeness [%] | 92.0 | 78.8 |
Redundancy | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 294 | Ammonium sulfate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |