1JLH

Human Glucose-6-phosphate Isomerase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LNLS BEAMLINE D03B-MX1
Synchrotron site	LNLS
Beamline	D03B-MX1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-07-01
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5450
Spacegroup name	P 21 21 21
Unit cell lengths	80.750, 108.021, 271.073
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.210 - 2.100
Rwork	0.197
R-free	0.24000 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dqr
RMSD bond length	0.008
RMSD bond angle	21.600 *
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.210	2.230
High resolution limit [Å]	2.100	2.100
Rmerge	0.111	0.399
Total number of observations	672976 *
Number of reflections	138362
<I/σ(I)>	6.7
Completeness [%]	99.7 *	99.4
Redundancy	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	Cordeiro, A.T., (2001) Acta Crystallogr., D57, 592. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8 (mg/ml)
2	1	reservoir	PEG10000	10 (%)
3	1	reservoir	HEPES	100 (mM)	pH7.5