1JL3
Crystal Structure of B. subtilis ArsC
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2001-06-08 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9999, 0.9700, 0.9500 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.940, 94.310, 118.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.800 * - 1.600 |
| R-factor | 0.226 |
| Rwork | 0.226 |
| R-free | 0.24100 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.800 * | 1.700 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.044 * | 0.300 * |
| Number of reflections | 74177 | |
| <I/σ(I)> | 19 | 4.6 |
| Completeness [%] | 97.3 * | 97.4 |
| Redundancy | 4 | 3.92 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 278 | PEG 4000, ammonium sulfate, sodium acetate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 278K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | sodium acetate | 0.1 (M) | |
| 2 | 1 | reservoir | ammonium sulfate | 0.2 (M) | |
| 3 | 1 | reservoir | polyethyleneglycol methyl | 30-35 (%) | |
| 4 | 1 | reservoir | dithiothreitol | 5 (mM) |
