1JKY
Crystal Structure of the Anthrax Lethal Factor (LF): Wild-type LF Complexed with the N-terminal Sequence of MAPKK2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-03-28 |
| Detector | MARRESEARCH |
| Spacegroup name | I 41 3 2 |
| Unit cell lengths | 330.700, 330.700, 330.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.600 - 3.900 |
| R-factor | 0.296 |
| Rwork | 0.296 |
| R-free | 0.31600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1j7n |
| RMSD bond length | 0.009 |
| Data reduction software | DENZO (DENZO) |
| Data scaling software | SCALEPACK (DENZO) |
| Phasing software | CCP4 ((MOLREP)) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.600 | 4.000 |
| High resolution limit [Å] | 3.900 | 3.900 |
| Number of reflections | 22906 | |
| <I/σ(I)> | 12.9 | 2 |
| Completeness [%] | 84.9 | 88.1 |
| Redundancy | 3.7 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 1.9M Ammonium sulfate, 0.2M Tris pH 8.0, 2mM EDTA |
